Comparison of methods for the quantification of albumin in serum and cerebrospinal fluid

Autores/as

  • Vanessa Pérez-del-Vallín Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez
  • José Alejandro Rodríguez-Pérez Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez
  • Alejandro Mirabal-Viel Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez
  • Christian Meijides-Mejías Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez
  • Jairo Lumpuy-Castillo Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez
  • William Castillo-González Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez
  • Alberto Juan Dorta-Contreras Laboratorio Central de Líquido Cefalorraquídeo (LABCEL) Universidad de Ciencias Médicas de la Habana, Facultad Miguel Enríquez

Palabras clave:

albumin, serum, cerebrospinal fluid

Resumen

Introduction: Albumin is the protein that marks the passage of cerebrospinal fluid proteins into the serum. Objective:  Determinate if the modified ELISA method is better than the bromocresol green method for serum in terms of specificity and sensitivity and in methods for quantifying cerebrospinal fluid. Materials and methods: Serum albumin was quantified by bromocresol green dye technique and by using the modified microalbuminuria method and for albumin in cerebrospinal fluid, the pyrogallol molybdate red complex  technique and the bromocresol green method were used. Results: Compared methods in serum, the modified ELISA method had a sensitivity of 100%, a specificity of 80% and the cut-off point was less than or equal to 58 g / L. In both fluids the bromocresol green method had a sensitivity of 72.7%, specificity of 60% and the cut-off point was greater than 34g / L. The areas under the ROC curves of the two methods did not show significant differences for p = 0.074. In the methods for cerebrospinal fluid the pyrogallol molybdate red complex had a sensitivity of 88.9% and a specificity of 90.5 and the cut-off point was less than or equal to 960mg / L and the bromocresol green method had a sensitivity of 66.7, a specificity of 85.7 and a cut-off point less than or equal to 700mg / L. The comparison between the ROC curves of the two methods studied was significant with p=0.138. Conclusions: The modified ELISA method is better than the bromocresol green method for serum in terms of specificity and sensitivity and in methods for quantifying cerebrospinal fluid albumin both methods do not show significant differences and have lower specificity and sensitivity than methods for quantifying albumin.

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Publicado

2020-01-28

Cómo citar

1.
Pérez-del-Vallín V, Rodríguez-Pérez JA, Mirabal-Viel A, Meijides-Mejías C, Lumpuy-Castillo J, Castillo-González W, et al. Comparison of methods for the quantification of albumin in serum and cerebrospinal fluid. Rev Cubana Inv Bioméd [Internet]. 28 de enero de 2020 [citado 30 de julio de 2025];38(5). Disponible en: https://revibiomedica.sld.cu/index.php/ibi/article/view/540