Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats

Autores/as

  • Basak Donertas Department of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. Florida
  • Wendi L Malphurs Department of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. Florida
  • David M. Baekey Department of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. Florida
  • David Julian Department of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. Florida
  • Jasenka Zubcevic Department of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. Florida

Palabras clave:

Hydrogen sulfide, Hypertension, Gut

Resumen

Introduction: Gut bacteria play a significant role in host homeostasis, and gut dysbiosis has been associated with many conditions including hypertension (HTN). In circulation, endogenously produced hydrogen sulfide (H2S) is an important freely-diffusing molecule that plays a role in modulation of neural, cardiovascular and immune systems. In view of this, reduced circulating levels of H2S have been shown in animal and human HTN. However, research to date has mainly focused on the H₂S endogenously produced by the host, while scarce evidence suggests that gut bacteria may also contribute to the overall levels of H₂S in host circulation. However, the role of gut bacteria-derived H2S in HTN has not been determined.

Objective: To investigate whether spontaneously hypertensive rat (SHR), an established model of HTN, has a disruption in the gut bacteria-derived H2S production.

Material and Methods: Bacterial DNA from fecal samples of adult male normotensive Wistar–Kyoto (WKY) and SHR was isolated for 16s bacterial genomic sequencing. WKY and SHR fecal and plasma samples were analyzed for H2S levels using modified methylene blue assay. Blood pressures were determined in all rats at several time points using tail cuff to confirm established HTN in the SHR. Data were expressed as mean±SEM. P<0.05 was considered statistically significant.

Results: We observed a significant reduction in the abundance of two H2S-producing gut bacteria in the SHR compared to WKY (Enterobacteriaceae: 1.423e-005±1.056e-005 vs. 0.0002329±7.853e-005, n=6, P<0.05; Clostridiaceae: 0.01134±0.00431 vs. 0.06408±0.01416, n=6, P<0.01). This decrease in the H2S-producing gut bacteria also reflected in a significant reduction in fecal H2S levels (SHR: 0±0.01703 AU vs. WKY: 0.094±0.03385 AU, n=5; P<0.05) and lower plasma H2S levels (SHR: 0.3265±0.07817 AU vs. WKY: 0.6850±0.1790 AU, n=2) in the SHR compared to the WKY.  

Conclusions: These results suggest that diminished gut bacterial production of H2S may contribute to the reduced H2S observed in host circulation in established HTN.

 

Keywords: Hydrogen sulfide; Hypertension; Gut

 

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Publicado

2020-01-28

Cómo citar

1.
Donertas B, Malphurs WL, Baekey DM, Julian D, Zubcevic J. Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats. Rev Cubana Inv Bioméd [Internet]. 28 de enero de 2020 [citado 30 de julio de 2025];38(5). Disponible en: https://revibiomedica.sld.cu/index.php/ibi/article/view/493

Número

Vol. 38 (2019): Suplemento Especial: II PanAmerican Congress of Physiological Sciences

Sección

COMUNICACIÓN BREVE

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