First ecuadorian report of qnrb19 and aac(6')-ib-cr genes in uropathogenic escherichia coli resistant to ciprofloxacin

Authors

  • Carlos Chiluisa-Guacho
  • Nairovys Gómez Martínez
  • Germania Elisabeth Vilema-Vizuete
  • Marise Dutra-Asensi

Keywords:

qnrB, aac(6')-Ib-cr, Escherichia coli, ciprofloxacin, uropathogenic resistant

Abstract

Escherichia coli is a bacterium found in the intestine of humans and other warm-blooded animals. Most of its strains are harmless and are part of the normal intestinal flora, playing an important role in digestion and vitamin synthesis. However, some strains can be pathogenic and cause diseases such as urinary tract infections, gastroenteritis, neonatal meningitis and septicemia.This study evaluated the presence of Plasmid Mediators of Quinolone Resistance (PMQR) in 156 clinical isolates of ciprofloxacin-resistant uropathogenic Escherichia coli in Quito-Ecuador, during 2011. It was found that 50.6 % of the isolates were resistant to ciprofloxacin, being more prevalent in hospitalized patients (75 %) than in outpatients (43.3 %). Genetic analysis revealed that 54.4 % of the resistant isolates carried the aac(6')-Ib-cr gene, and 64.5 % the qnrB gene, with the qnrB19 allele present in 100 % of the strains analyzed. The qnrA, qnrC, qnrD and qnrS genes were not detected. In addition, 36.7 % of the isolates showed co-oexpression of qnrB19 and aac(6')-Ib-cr genes, associated with high levels of resistance. The distribution of phylogenetic groups was 22.8 % in group A, 15.5 % in group B1, 48.1 % in group B2 and 13.9 % in group D. This study is the first report of the presence of qnrB19 and aac(6′)-Ib-cr genes in Ecuador, highlighting the importance of monitoring ciprofloxacin resistance and PMQR genes in uropathogenic E. coli.

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Published

2024-07-19

How to Cite

1.
Chiluisa-Guacho C, Gómez Martínez N, Vilema-Vizuete GE, Dutra-Asensi M. First ecuadorian report of qnrb19 and aac(6’)-ib-cr genes in uropathogenic escherichia coli resistant to ciprofloxacin. Rev Cubana Inv Bioméd [Internet]. 2024 Jul. 19 [cited 2025 Dec. 8];43. Available from: https://revibiomedica.sld.cu/index.php/ibi/article/view/3416