Nanogels as prospective Biomaterial: Radio-induced Synthesis, Characterization, and Biological Assays

Autores/as

  • Adrian Alejandro Ges Naranjo Universidad de la Habana
  • Herlys Viltres Cobas Centre of Research in Applied Science and Advanced Technology, Legaria-Unit National Polytechnic Institute
  • Liudy García Hernández Centre for Technological Applications and Nuclear Development
  • Liena Ponce Rey Faculty of Biology, University of Havana.
  • Yuri Aguilera Corrales Higher Institute of Technology and Applied Sciences, University of Havana
  • Isabelle Luz Laboratory of Biochemistry and Protein Chemistry, University of Brasilia
  • Katyelle Botelho Laboratory of Biochemistry and Protein Chemistry, University of Brasilia
  • Wagner Fontes Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

Palabras clave:

biomaterial, citotoxicidad, radiación gamma, nanogel, neutrófilo.

Resumen

Nowadays, there is a growing interest in biodegradable polymers-based materials due to their diverse application in the biomedical field. Most studied systems involve biocompatible micro and nanodevices, such as liposomes, dendrimer, micelles or polymeric nanogels. The use of Radiation Technology, specifically gamma radiation, to produce micro and nanogels raises the possibility to obtain higher purity products, an important feature for biomedical and pharmaceutical applications. The radio-induced synthesis, characterization, cytotoxicity evaluation, and immunological response of nanogels are described in this study. Nanogel synthesis was performed in the absence of oxygen using aqueous polyvinylpyrrolidone solutions. Crosslinking reactions were carried out at 25 °C in a gamma irradiation chamber with a 60Co source. Nanogels properties were analysed by Scanning Electron Microscopy, Attenuated Total Reflection-Fourier Transform Spectroscopy, Dynamic Light Scattering, and Viscosimetry. The cytotoxicity and immunological response were evaluated by MTT test and analysis of the neutrophil respiratory burst. The results showed that nanogels formation strongly depends on the total absorbed dose. The nanogels have an elliptical shape and their chemical structure is similar to the initial polymer. The nanogels are biocompatible and promote a low-intensity neutrophil activation, similar to the well-characterized biomaterial TiO2, suggesting their potential biomedical uses.

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Biografía del autor/a

Adrian Alejandro Ges Naranjo, Universidad de la Habana

Profesor e investigador

Departamento de Radioquímica

Herlys Viltres Cobas, Centre of Research in Applied Science and Advanced Technology, Legaria-Unit National Polytechnic Institute

Researcher

Liudy García Hernández, Centre for Technological Applications and Nuclear Development

Full Researcher

Liena Ponce Rey, Faculty of Biology, University of Havana.

Assistant professor

Department of Microbiology and Virology

Yuri Aguilera Corrales, Higher Institute of Technology and Applied Sciences, University of Havana

Full professor

Full researcher

Department of Radiochemistry

Isabelle Luz, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

Master student at University of Brasilia

Katyelle Botelho, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

Master student at University of Brasilia

Wagner Fontes, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

Full professor

University of Brasilia

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Publicado

2021-05-28

Cómo citar

1.
Ges Naranjo AA, Viltres Cobas H, García Hernández L, Ponce Rey L, Aguilera Corrales Y, Luz I, et al. Nanogels as prospective Biomaterial: Radio-induced Synthesis, Characterization, and Biological Assays. Rev Cubana Inv Bioméd [Internet]. 28 de mayo de 2021 [citado 15 de julio de 2025];40(5). Disponible en: https://revibiomedica.sld.cu/index.php/ibi/article/view/1176