The effect of titanium alloy and stainless steel implants on immunological responses by analysis of NF-κB/ p65, NF-κB1/p50 profiles and the Tregs

Authors

  • Sibel Akyol Istanbul University Cerrahpasa. Cerrahpasa Medical Faculty. Department of Physiology. Istanbul, Turkey. University of Technology Sydney. Faculty of Science. School of Life Sciences. Sydney. Australia
  • Murat Hanci Istanbul University Cerrahpasa. Cerrahpasa Medical Faculty. Department of Neurosurgery. Istanbul.Turkey
  • Besim Ben-Nissan University of Technology Sydney. Faculty of Science. School of Life Sciences. Sydney. Australia

Keywords:

Titanium alloy implants, stainless steel implants, immunological response, NF-κB/ p65, NF-κB1/p50, Tregs

Abstract

Introduction: A comprehensive understanding of the tissue-biomaterial interactions at a cellular level is required to explain the immune responses of numerous implant mediated complications and help to improve biomaterial design and use.

Objectives: To research the effect of titanium alloy and stainless steel implants on immunological responses in rats by analysis of NF-κB/ p65, NF-κB1/p50 profiles in the activation of inflammatory signaling pathways and the role of Tregs. To minimize the inflammatory response of host-formed bio-material implants is our main goal.

Materials and Methods: In this study, 39 Wistar albino male rats were divided into three groups with 13 rats each resulting in Group I (n: 13, sham), Group II (n: 13, Ti alloy rods), and Group III (n: 13, SS alloy rods). The NF-κB/ p65, NF-κB1/p50 and CD4+CD25+Foxp3+ (Tregs) in the blood were analyzed on days 7, 14 and 28 using ELISA and Flow cytometry.

Results: Tregs level were lower in the stainless steel (SS) alloy compared to the sham and Titanium (Ti) alloy. NF-κB/ p65 (RelA) levels in the SS alloy showed a significant increase on all days in comparison with the sham and Ti alloy. NF-κB1 (p50) in the SS alloy was a significant increase on the 14th and 28th day. When the Ti alloy was compared with the SS alloy, NF-κB/ p65 (RelA) and NF-κB1 (p50) levels were significantly lower levels.

Conclusions: Both the Ti alloy and SS alloy group implantation effects CD4+CD25+Treg cells in different ways. This work suggests that NF-κB/ p65, NF-κB1/p50 have excellent potential as a therapeutic target in the prevention of adverse reactions to metal, especially for controlling the inflammation after the implantation. In this application target can be NF-kB and for this IKK molecule inhibitors can be used or it can be done by the stabilization of IkB proteins.

 

Keywords: Titanium alloy implants, stainless steel implants, immunological response,  NF-κB/ p65, NF-κB1/p50, Tregs

 

 

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Published

2020-01-28

How to Cite

1.
Akyol S, Hanci M, Ben-Nissan B. The effect of titanium alloy and stainless steel implants on immunological responses by analysis of NF-κB/ p65, NF-κB1/p50 profiles and the Tregs. Rev Cubana Inv Bioméd [Internet]. 2020 Jan. 28 [cited 2025 Aug. 31];38(5). Available from: https://revibiomedica.sld.cu/index.php/ibi/article/view/458

Issue

Vol. 38 (2019): Suplemento Especial: II PanAmerican Congress of Physiological Sciences

Section

SHORT COMMUNICATIONS

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