Influence of NeuroEPO on the fetal pancreas of rats with placental insufficiency
Keywords:
Páncreas; insuficiencia placentaria; eritropoyetinaAbstract
Introduction: placental insufficiency is the most common cause of intrauterine growth retardation. In the fetus with IUGR, hypoxia and malnutrition can cause alterations in pancreatic development. It is known that NeuroEPO, due to its therapeutic properties, protects tissues under hypoxic conditions.
Objectives: to evaluate the influence of human erythropoietin with low sialic acid content (NeuroEPO) on the fetal pancreas of rats in a model of placental insufficiency.
Method: pregnant Wistar rats underwent ligation of the right uterine artery on day 16 of gestation. On the same day, half of the rats received NeuroEPO (0.5 mg/kg/day subcutaneously for three days) and the rest received a placebo. On day 20 of gestation, the offspring were divided into four groups based on the uterine horn in which they were located: a Control group (n=35), an IUGR group (n=20), a Control NeuroEPO group (n=38), and an IUGR NeuroEPO group (n=24). Fetuses and their placentas were weighed. Fetal length and cranial diameters were measured. A random sample of 5 fetal pancreases per group was taken. Histological variables were studied in this organ using haematoxylin-eosin and Gomori staining.
Results: fetuses with IUGR exhibited decreased body weight, and their placentas were less efficient. In the pancreas, a reduction in the density of the islets of Langerhans and beta cells was observed. In the IUGR group treated with NeuroEPO, these variables were similar to those in the control group.
Conclusions: administration of this molecule improved fetal weight and promoted proper pancreatic development, possibly due to its cytoprotective effects.
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