Antibacterial activity of a Peruvian propolis ethanolic extract against <i>Streptococcus mutans</i>

Authors

Keywords:

antibacterial agent, propolis, Streptococcus mutans, terpenes

Abstract

Introduction: Due to their important antimicrobial properties, propolis by-products are potentially useful for the prevention and treatment of dental caries.

Objective: Evaluate the antibacterial activity of a Peruvian propolis ethanolic extract against Streptococcus mutans.

Methods: The propolis ethanolic extract was obtained by maceration in 70% alcohol for 15 days. The extract was diluted in distilled water to obtain concentrations of 75%, 50% and 25%. Antibacterial activity was evaluated by the disk diffusion test in brain heart agar medium inoculated with S. mutans ATCC® 25175™. Chlorhexidine (CHX) 0.12% was used as control. The Petri plates were incubated for 48 hours at 37ºC in microaerophilic conditions. The inhibition haloes were then measured with a Vernier caliper.

Results: All the concentrations of the propolis ethanolic extract displayed antibacterial activity against S. mutans: 25% = 17.582 ± 2.578 mm; 50% = 16.906 ± 1.892 mm; 75% = 16.881 ± 2.013 mm; 100% = 17.201 ± 1.305 mm. However, values were lower than those of 0.12% CHX: 24.543 ± 2.486 mm (p < 0.05). According to the Duraffourd scale, S. mutans was sensitive (+) and very sensitive (++) to all propolis ethanolic extract concentrations, and highly sensitive to 0.12% chlorhexidine (+++) (p < 0.05).

Conclusions: The Peruvian propolis ethanolic extract displays significant antibacterial activity against S. mutans. Such activity was evaluated as sensitive and very sensitive.

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Author Biographies

Marco Antonio Sánchez-Tito, Universidad Privada de Tacna

Cirujano Dentista, Especialista en Ortodoncia y Ortopedia Maxilar, Maestro en Investigación Científica e Innovación, Doctorando en Estomatología

Jackeline Luciana Checalla Collatupa, Práctica privada

Cirujano Dentista

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Published

2021-10-27

How to Cite

1.
Sánchez-Tito MA, Checalla Collatupa JL. Antibacterial activity of a Peruvian propolis ethanolic extract against <i>Streptococcus mutans</i>. Rev Cubana Inv Bioméd [Internet]. 2021 Oct. 27 [cited 2025 Jul. 12];40(3). Available from: https://revibiomedica.sld.cu/index.php/ibi/article/view/1121

Issue

Vol. 40 No. 3 (2021): Julio-Septiembre

Section

ARTÍCULOS ORIGINALES

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