Neuroimmunological approach for evaluation of viral measles, mumps and rubella triple vaccineIntroduction: During the neuroinflammatory processes there are a poly-specific and polyclonal activation in the cerebrospinal fluid. It means that there can be quantified antibodies against all the components of the vaccines may have received.
Objective: To evaluate the immune response against measles, mumps and rubella in vaccinated pediatric patients.
Methods: All the serum and cerebrospinal fluid (CSF) paired samples from pediatric patients with neurological symptoms, that were submitted to hospitals from Havana City and were performed a lumbar puncture, were collected. Serum and CSF IgG, albumin were measured by immune-diffusion techniques using NOR and LC Partigen Immunoplates (Siemens, Marburg) and specific antibodies against measles, mumps and rubella were quantified by ELISA kits (Enzygnost, Siemens, Marburg). Reibergrams were employed in order to determine if there was IgG intrathecal synthesis. Later on, antibody index against the specific virus were calculated.
Results: In all the neuroimmune inflammation process were found antibody index against measles, mumps and rubella in a different ample confidence variation among the different virus. Antibodies against mumps are significantly different from the other ones. It could be due to a natural different immune response or due to a deficient vaccine quality lot. Also it was possible to identify six pediatric patients that had no immune antibody index at all. It coincides with a transient hypogammaglobulinemia of infancy in such patients.
Conclusions: This neuroimmunological approach can be used to evaluate the immune status in pediatric population.
William Castillo González, Mileydis Cruz Quevedo, José Alejandro Rodríguez Pérez, Eneida Barrios Lamoth, Cristóbal González Losada, Alberto Juan Dorta Contreras
IgG anti-Toxoplasma gondii antibody index as a measure of the naturally-acquired immune intrathecal response in a neuroepidemiological studyIntroduction: The presence of IgG anti-Toxoplasma gondii has been used as an indicator to measure the naturally-acquired immune intrathecal response due to the polyclonal and polyspecific intrathecal response developed in neuroinflammation processes. The absence of anti- Toxoplasma gondii antibodies in a subject who had been exposed to the parasite could be seeded as immunocompromised. The aim of the study is determine cases of autoimmunity and immunodeficiency.
Objective: Demonstrate whether the IgG anti-Toxoplasma gondii antibody index can be used as an indicator of a population immune status seen through patients with neuroinflammatory processes.
Methods: Cerebrospinal fluid (CSF) and serum samples were taken from 64 pediatric patients with intrathecal synthesis of antibodies and IgG anti-Toxoplasma gondii antibody indexes (AI) were determined.
Results: The sample was divided in three intervals according to Hansotto Reiber investigations (·0.6; 0.6-1.5; ·1.5) and a mean interval ±1SD between 0.23 and 1.12. The groups were quite similar regarding to clinic and demographic characteristics; there were statics differences on anti-Toxoplasma gondii AI (p<0.01) and the presence of domestic animals (p=0.04). In the group with AI·1.5, the 83.3% had positive the Measles-Rubella-Zoster reaction, indicative of active autoimmune disease. On the group with AI·0.6 were analyzed six different variables trying to find possible cases with immunodeficiencies: risk factors to contract toxoplasmosis; subjective clinical of immunocompromise; a test to detect immunodeficiencies; response against vacunal antigens and humoral response determined by IgG levels in serum. The immunodeficiencies test was the variable with higher statistical significance (p= 0.047).
Conclusions: The conclusion was that is possible to find subjects with autoimmune disorder and other ones with immunocompromise through the IgG anti- Toxoplasma gondii index by the developed investigation strategy.
Mileydis Cruz Quevedo, William Castillo González, Alberto Juan Dorta Contreras
Eneida Barrios-Lamoth, Alberto Juan Dorta-Contreras
Elementos 1 - 3 de 3

Consejos de búsqueda:

  • Los términos de búsqueda no distinguen entre mayúsculas y minúsculas
  • Las palabras comunes serán ignoradas
  • Por defecto, sólo aquellos artículos que contengantodos los términos en consulta, serán devueltos (p. ej.: Y está implícito)
  • Combine múltiples palabras conO para encontrar artículos que contengan cualquier término; p. ej., educación O investigación
  • Utilice paréntesis para crear consultas más complejas; p. ej., archivo ((revista O conferencia) NO tesis)
  • Busque frases exactas introduciendo comillas; p.ej, "publicaciones de acceso abierto"
  • Excluya una palabra poniendo como prefijo - o NO; p. ej. -política en línea o NO política en línea
  • Utilice * en un término como comodín para que cualquier secuencia de caracteres concuerde; p. ej., soci* moralidad hará que aparezcan aquellos documentos que contienen "sociológico" o "social"