Inflammation Modulation with Methanol Zea Mays Cob Husk on an Experimental Model in Albino Rats
Palabras clave:
Inflammation, methanol zea mays cob husk, experimental model, ratsResumen
Introduction: The anti-inflammatory effect of Zea mays cob husk extract may be attributed to the presence of saponins, tannins and polyphenols. Objective: to evaluate the modulatory effect on carrageenan induced inflammation of the methanolic extract of Zea mays cob husk in Albino rats. Methods: The carrageenan-induced paw edema model of acute inflammation was carried out to 30 Albino rats which were divided into five groups, two controls (negative and positive) and three experimental. 9.45% maize cob husks methanol extract was previously obtained by soxhlet extraction and the phytochemical screening was done. Acute toxicity studies were conducted in 12 rats. A single oral methanol plant extract (100mg/Kg, 200mg/Kg or 400mg/Kg) were administered to the experimental groups. Indomethacin 10mg/Kg was used as positive control. The paw volume up to the tribiotural articulation was measured at 0, 1, 3, and 6th hours. Inflammation was expressed as an increase in paw volume due to carrageenan injection and the percentage inhibition produced by the extracts was calculated to assess the anti-inflammatory activity. ANOVA was used to determine the statistical differences between the treatment groups considering a P value less than 0.05 significant. The research was conducted in conformance with the Ethical Principles for the use of animals in research with approval by the Ethical Research Committee of the Institution. Results: saponins, reducing sugars, steroids, tannins and polyphenols were present in the methanol Zea mays cob husk extract. Doses below 5000mg/Kg were considered safe. Significant anti-inflammatory activity was shown at 400mg/Kg after the sixth hour. Conclusions: The anti-inflammatory effect of Zea mays cob husk extract may be attributed to the presence of saponins, tannins and polyphenols. Percentage inhibition of inflammation by the extracts showed dose and time dependence. The antinflammatory activity was found significant for safe concentrations of the plant extract.
Descargas
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Aquellos autores/as que tengan publicaciones con esta revista, aceptan los términos siguientes: Los autores/as conservarán sus derechos de autor y garantizarán a la revista el derecho de primera publicación de su obra, el cuál estará simultáneamente sujeto a la Licencia de reconocimiento de Creative Commons (CC-BY-NC 4.0) que permite a terceros compartir la obra siempre que se indique su autor y su primera publicación esta revista. Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista. Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos telemáticos institucionales o en su página web) antes y durante el proceso de envío, lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).
Como Revista Cubana de Investigaciones Biomédicas forma parte de la red SciELO, una vez los artículos sean aceptados para entrar al proceso editorial (revisión), estos pueden ser depositados por parte de los autores, si estan de acuerdo, en SciELO preprints, siendo actualizados por los autores al concluir el proceso de revisión y las pruebas de maquetación.